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. 2014 Feb 6;10(2):e1004110. doi: 10.1371/journal.pgen.1004110

Figure 2. Conditional inactivation of the Nsun4 gene in heart and skeletal muscle.

Figure 2

A. Decreased lifespan of mice with a muscle-specific knockout of Nsun4. Survival curve for control (L/L; n = 20; open squares) and mutant mice (L/L, cre; n = 16; filled squares). B. Heart- to body-weight ratio in control (L/L; open squares) and mutant mice (L/L, cre; filled squares). Number of analyzed animals at 5 weeks L/L n = 6, L/L, cre n = 6; at 10 weeks L/L n = 7, L/L, cre n = 8; at 15 weeks L/L n = 5, L/L, cre n = 7; at 20 weeks L/L n = 12, L/L, cre n = 12. Data are represented as mean +/− SEM. *, p<0.05; **, p<0.01; ***p<0.001. Student's t test. C. BN-PAGE analysis of levels of assembled respiratory chain complexes in control (L/L) and knockout (L/L, cre) mice at different ages. D. Western immunoblotting of steady-state levels of NSUN4 in heart mitochondrial extracts from control (L/L) and knockout (L/L, cre) mice at different ages. VDAC was used as a loading control.