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. 2014 Feb 6;10(2):e1004110. doi: 10.1371/journal.pgen.1004110

Figure 3. Mitochondrial translation and ribosome assembly in Nsun4 knockout hearts.

Figure 3

A. Pulse-labeling of mitochondrial translation products in isolated heart mitochondria from 20 weeks-old control (L/L) and knockout mice (L/L, cre). The Coomassie-stained gel is a loading control. Known mitochondrial polypeptides are indicated. B. Western blot analysis of steady-state levels of mitochondrially and nucleus-encoded OXPHOS proteins in mitochondria from control and knockout hearts at different ages. VDAC was used as a loading control. *, cross reaction. C. Analysis of mitoribosomal assembly by sucrose gradient ultracentrifugation of heart mitochondrial extracts from control (L/L) and mutant (L/L, cre) mice. Sedimentation of 28S (SSU, fraction 6), 39S (LSU, fraction 8) and 55S (assembled ribosomes, fraction 10) was determined by western blot analysis using MRPS15- and MRPL13-specific antibodies. D. Western blot analysis of steady-state levels of MRPL13 and MRPS15 in heart mitochondrial extracts from control (L/L) and knockout (L/L, cre) mice at different ages. VDAC was used as a loading control.