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Effect of endothelium removal on vasoconstriction responses. A, Mechanical removal of endothelium with a human hair and viability test showing no relaxation response to acetylcholine. B, Endothelium-denuded arteries from both MetS and I-MetS groups still presented significantly increased vasoconstriction responses compared to controls. Data are presented as mean ± SEM, n = 4–5 mice/group, **P <0.01, ***P < 0.001.
