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Flow cytometry assessment of neutrophil phagocytosis. (A) Trypan blue quenching of the extracellular fluorescence of bound bacteria and phagocytosed bacteria by neutrophils after incubation at 37°C for 60 min were evaluated by flow cytometry. (B) The KP-M1 bacteria were significantly more susceptible to phagocytosis by neutrophils after incubation with 10 or 30 μg/mL sodium salicylate (SAL).
