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. 2014 Feb 6;9(2):e86778. doi: 10.1371/journal.pone.0086778

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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In both fundus (top panels) and CCP (bottom panels), immunoreactivity for myosin Va (green) is present in nerve fibers that are immunoreactive for nNOS (red). Co-localization (yellow) is present in projected image. Western blot on right shows positive bands for myosin Va and nNOS in total protein lysate (TP) in lane 1. In lane 2, samples were immunoprecipitated for nNOS and blotted for myosin Va to demonstrate molecular interaction between these proteins in fundus (top panel) and CCP (bottom panel).