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. 2014 Feb 6;9(2):e87661. doi: 10.1371/journal.pone.0087661

Figure 6. Regulation of Oip5 in 3T3-L1 adipocytes.

Figure 6

A, Change of Oip5 mRNA level during 3T3-L1 adipocytes differentiation. **P<0.01;***P<0.001, compared to day 0. B, Oip5 mRNA level of 3T3-L1 adipocytes on day 9 and 21 after the induction of differentiation. C, Effect of tumor necrosis factor-α (TNF-α), insulin and H2O2 on Oip5 mRNA levels in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with or without 10 ng/mL of TNF-α, 5 µg/mL of insulin, and 50 µM of H2O2 for 24 hrs. D, Effect of peroxisome proliferator-activated receptor-γ (PPARγ) agonists on Oip5 mRNA levels. 3T3-L1 adipocytes were treated with 1 µM of pioglitazone (Pio) and rivoglitazone (Rivo) for 24 hrs. Oip5, Opa-interacting protein 5; TNF-α, tumor necrosis factor-α; Cont, control; Pio, pioglitazone; Rivo, rivoglitazone. Values are mean ± SE; n = 3–6 for each group. **P<0.01;***P<0.001.