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. 2014 Feb 6;9(2):e87694. doi: 10.1371/journal.pone.0087694

Table 1. PCR primer sequences and annealing temperature.

Gene Upper Sequence Lower Sequence Product(bps) Temperature(°C) Reference
JAK1 CGGAACCAATGACAACGAACAGTC CCAAGGTAGCCAGGTATTTCACC 473 63 [50]
STAT3 CTTGGGCATCAATCCTGTGG TGCTGCTTGGTGTATGGCTCTAC 514 63 [50]
STAT5a ATTACACTCCTGTACTTGCGA GGTCAAACTCGCCATCTTGG 212 58.4 [51]
STAT5b TCCCCTGTGAGCCCGCAAC GGTGAGGTCTGGTCATGACT 321 62 [51]
TNFα CATCTTCTCAAAATTCGAGTGACAA TGGGAGTAGACAAGGTACAACCC 175 45 [52]
IL6 ATGGATGCTACCAAACTGGAT TGAAGGACTCTGGCTTTGTCT 139 45 [52]
Actin TGTGATGGTGGGAATGGGTCAG TTTGATGTCACGCACGATTTCC 514 58 [53]