Table 1. PCR primer sequences and annealing temperature.
Gene | Upper Sequence | Lower Sequence | Product(bps) | Temperature(°C) | Reference |
JAK1 | CGGAACCAATGACAACGAACAGTC | CCAAGGTAGCCAGGTATTTCACC | 473 | 63 | [50] |
STAT3 | CTTGGGCATCAATCCTGTGG | TGCTGCTTGGTGTATGGCTCTAC | 514 | 63 | [50] |
STAT5a | ATTACACTCCTGTACTTGCGA | GGTCAAACTCGCCATCTTGG | 212 | 58.4 | [51] |
STAT5b | TCCCCTGTGAGCCCGCAAC | GGTGAGGTCTGGTCATGACT | 321 | 62 | [51] |
TNFα | CATCTTCTCAAAATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC | 175 | 45 | [52] |
IL6 | ATGGATGCTACCAAACTGGAT | TGAAGGACTCTGGCTTTGTCT | 139 | 45 | [52] |
Actin | TGTGATGGTGGGAATGGGTCAG | TTTGATGTCACGCACGATTTCC | 514 | 58 | [53] |