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. 2014 Feb 6;9(2):e87893. doi: 10.1371/journal.pone.0087893

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© 2014 Xu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Flow cytometry analysis of CD133 expression in heterotypic hybrids and respective parental lung cancer cells. Percentages of CD133⁺ cells are indicated in control antibody and specific antibody-stained cells. Grey and black line indicated negative control and sample, respectively. (B) The expression levels of the mRNAs encoding CD133, Nanog, BMI1, Notch1, ALDH1, Sox2 and OCT4 in spontaneously-formed A549/MSC, H460/MSC and SK-MES-1/MSC heterotypic hybrids relative to respective parental lung cancer cells as determined by QRT-PCR. GAPDH mRNA was used to normalize the variability in template loading. The data are reported as mean ± SEM.