Figure 2. Elastomer mask cell migration assay. (A–C) Sequence of images of one cell-free area in a PDGF-treated culture showing migration of VSMCs at T0, T24, and T48. (D–F) Image sequence from panels A-C with the leading edge of migratory cells traced for analysis of the proportion of the cell-free area invaded. Scale bar = 250 µm for all panels.