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. 2013 Dec 2;7(6):469–475. doi: 10.4161/cam.27294

Table 1. Reagents and materials.

Name of reagent/material Company Catalog number Comments
Sylgard 184 Ellsworth Adhesives 184 SIL ELAST KIT 0.5KG  
Recombinant rat PDGF-BB R&D Systems 520-BB-050  
Dulbecco’s Modified Eagle’s Medium (DMEM) Invitrogen 12100-061  
Lactalbumin hydrolysate Invitrogen 895086IP  
1X antibiotic-antimycotic Invitrogen 15240096  
Heat-inactivated fetal bovine serum. Invitrogen 11150  
BD Falcon 24-well culture plate VWR 21100-004 6- or 12-well culture plates or individual petri dishes of any size also will work.
Miltex biopsy punches (2mm) with ejector VWR 95039-098 Also available in other diameters up to 6mm
Forceps, Dumont Style 2 Electron Microscopy Sciences 0103-2-PO Straight or curved forceps will work according to user preference
Electron microscopy embedding molds (polyethylene) Electron Microscopy Sciences 70904-01 Other sizes and shapes of molds also available. Silicone rubber molds not recommended.
Tissue-Tek Cryomold, biopsy molds (vinyl) Electron Microscopy Sciences 62534-10 Other sizes and shapes of molds also available. Silicone rubber molds not recommended.