Skip to main content
. 2014 Feb 6;10(2):e1003906. doi: 10.1371/journal.ppat.1003906

Figure 1. Delayed worm expulsion in CD28 deficient mice during primary N. brasiliensis infection.

Figure 1

Wild-type (C57BL/6) and CD28−/− mice were infected with 500 L3 N. brasiliensis and killed 9 and 12 days post-infection. (A), Schematic showing experimental set-up. (B), Intestinal worm burdens were quantified. (C), Goblet cells per villi were enumerated. (D), PAS staining of mucus producing goblet cells in the intestinal tissue. Cytokine production by total mesenteric lymph node cells collected 9 days (E) or 12 days (F) post-infection was determine by ELISA after re-stimulation of total cells in media or with 20 µg/ml α-CD3 for 72 h at 37°C. (G), Serum antibody titres of N. brasiliensis specific IgG1, IgG2a, IgG2b and total IgE were determined by ELISA. Data are representative of two independent experiments. n = 4–6 mice per group. *P<0.05, **P<0.01, and ***P<0.001 vs C75BL/6 mice using two-tailed Mann-Whitney non-parametric Student's t-test or Two-Way ANOVA with Bonferroni's post test.