Figure 3. CD28 is necessary for development of T_FH cells and optimal activation of CD4⁺ T cells.

Single cell suspension was prepared from mediastinal lymph node and cells were stained for flow cytometric analysis. (A), Absolute numbers of CD4⁺ T cells in the lymph node. (B), Absolute numbers of CD4⁺CXCR5⁺, CD4⁺CXCR5⁺ICOS⁺ and CD4⁺ICOS⁺ T cells recruited to the mediastinal lymph node. (C) Total number of T cell subsets infiltrating the draining lymph node. T cells subsets were differentiated based on the following markers: naive (CD4⁺CD44^loCD62L^hi), central memory (CD4⁺CD44^hiCD62L^hi) and effector memory (CD4⁺CD44^hiCD62L^lo) T cells. Data represents two independent experiments. n = 5–6 mice per experiment. *P<0.05, **P<0.01, and ***P<0.001 vs C57BL/6 mice using One-Way ANOVA with Bonferroni's post test.