Fig. 4.

In vivo anti-tumor effects of BBR on the Ham-1 allograft hamster model. 5×10⁵ cells of Ham-1 were injected intradermally into the dorsal skin of hamster (n=10/group). 10 mg/kg of BBR were given orally every day for 21 days. Equal volumes of drinking water were given to the control group. (A) Average weights of hamsters were compared. (B) Tumor weights were determined and representative tumors from control and BBR groups are shown (small inset). (C) The proliferative index (PCNA) and expressions of cell cycle control proteins (cyclin D1 and cyclin E) of Ham-1 cells in tumor tissues were assessed by immunohistochemistry. Pictures were taken under light microscope (×400). Bar=20 µm. (D) IHC scores were determined by 2 independent assessors and presented as mean±SD. ^*P<0.05, ^**P<0.001, vs the control group.