FIGURE 1.

Hepatic CYP2D6 is induced in Tg-CYP2D6 mice during pregnancy. A, liver tissues of Tg-CYP2D6 mice were collected at different time points during gestation. mRNA expression levels of CYP2D6 and Cyp2d22 were determined by qRT-PCR. B, protein levels of CYP2D6 in the liver tissues were determined by using Western blotting. A representative image of Western blot is shown including a liver tissue sample from wild-type (WT) mice (top), and the band intensities were quantified (bottom). C, microsomes were prepared from the liver tissues of Tg-CYP2D6 or WT mice, and CYP2D6 phenotyping was performed using debrisoquine (200 μm) (n = 4, mean ± S.D.; ***, p < 0.001 one-way ANOVA versus virgin).