FIGURE 3.

Pregnancy-mediated CYP2D6 induction was abrogated in Hnf4α(−/wt) mice. A, liver tissues were collected from Hnf4α(wt/wt) and Hnf4α(−/wt) mice at pre-pregnancy. HNF4α protein expression levels were determined by a Western blot (bottom). The signal intensity was measured by densitometry and expressed relative to that of β-actin (top) (n = 4, mean ± S.D.; ***, p < 0.001, Student's t test versus Hnf4α(wt/wt) mice). B and C, mRNA levels of CYP2D6 (B) and Shp (C) in the liver tissues were determined by qRT-PCR (n = 4, mean ± S.D.; **, p < 0.01; ***, p < 0.001, one-way ANOVA versus virgin). D, ChIP assays were performed in liver tissues collected at different gestational time points (n = 4, mean ± S.D.; *, p < 0.05, one-way ANOVA versus virgin).