FIGURE 3.

Ectopic expression of Rbm24 increases whereas knockdown of Rbm24 decreases the level of p21 protein. A–C, MCF7, HCT116, and HCT116 (p53^−/−) cells were uninduced (−) or induced (+) with 0.5 μg/ml tetracycline to express Rbm24 for 48 h. The levels of Rbm24, p21, and actin proteins were then measured by Western blot analysis. D, H1299 cells were transiently transfected with a control vector or a vector expressing Rbm24 for 48 h. The protein levels of Rbm24, p21, and actin were determined by Western blot analysis. E and F, H1299 (E) and HCT116 (p53^−/−) (F) cells were transduced with a lentivirus expressing a control luciferase shRNA (shluc) or Rbm24 shRNA (shRbm24) and selected by puromycin (1 μg/ml) for 3 days prior to Western blot analysis.