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. 2013 Dec 19;289(6):3164–3175. doi: 10.1074/jbc.M113.524413

FIGURE 4.

FIGURE 4.

Ectopic expression of Rbm24 increases whereas knockdown of Rbm24 decreases the level of p21 mRNA. A–C, MCF7 (A), HCT116 (B), and HCT116 (p53−/−) (C) cells were uninduced (−) or induced (+) with 0.5 μg/ml tetracycline to express Rbm24 for 48 h. The levels of Rbm24, p21, and actin transcripts were measured by RT-PCR analysis. D, MEFs (p53−/−;RNPC1−/−) were transiently transfected with a control vector or a vector expressing Rbm24 for 48 h. RT-PCR analysis was used to determine the mRNA levels of Rbm24, p21, and actin. E and F, H1299 (E) and HCT116 (p53−/−) (F) cells were transduced with a lentivirus expressing a control luciferase shRNA (shluc) or Rbm24 shRNA (shRbm24) and selected by puromycin (1 μg/ml) for 3 days. The levels of Rbm24, p21, and actin transcripts were measured by RT-PCR analysis. G, MEFs (p53−/−;RNPC1−/−) were transiently transfected with a control or Rbm24 siRNA for 72 h. The levels of Rbm24, p21, and actin transcripts were determined by RT-PCR analysis.