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. 2013 Dec 16;289(6):3288–3293. doi: 10.1074/jbc.M113.518282

FIGURE 3.

FIGURE 3.

MEF2A is a target of SENP2. A, C2C12 cells were transfected with the SUMO-1, MEF2A, and MEF2Am plasmids, as indicated, and luciferase activity was measured 24 h after transfection. The data are presented as mean ± S.D. of three independent experiments. B, C2C12 cells were transfected with MEF2A, HA-SUMO-1, FLAG-SENP2, or SENP2m, as indicated. MEF2A proteins were pulled down by MEF2A antibody from these cell lysates. Bound proteins were blotted with anti-HA (top panel) or anti-MEF2A antibody (center panel). Cell lysate (Input) was immunoblotted (IB) with anti-HA antibody (bottom panel). IP, immunoprecipitation. C, the proteins extracted from Senp2+/+ (WT) and Senp2−/− (Mut) MEF cells were pulled down with anti-MEF2A antibody and immunoblotted with anti-SUMO-1 (top panel) or anti-MEF2A antibody (center panel). Cell lysate was immunoblotted with anti-SUMO-1 antibody (bottom panel).