TABLE 2.

List of primers used in this study

Primer name	Primer sequence
GPI2 FP	5′GCGGAGCTCATGGAAGAAATACATATAAGCTCT3′
GPI2 RP	5′GCGAAGCTTTCAGCTTTGTATACTTGACTTCAT3′
GPI2 5′hz RP	5′GCGAGATCTGTCCCATTGCTTCTCATATATT3′
GPI23′hz FP	5′GCGGTCGACACATTAGACGTATTTTTGTTTG3′
GPI2-ARG4 FP	5′ATGGAAGAAATACATATAAGCTCTTCCATTTTGCAAGATAGTGATTCAGCTCCCCCAAACCATTTTCAGTGTGGAATTGTGAGCGGATA3′
GPI2-ARG4 RP	5′TCAGCTTTGTATACTTGACTTCATTAACTTTGGTTTCGCCACATCCCATGGTCCTTGTATCTCGTTTTTTTTCCCAGTCACGACGTT3′
GPI2 Up FP	5′AAGTATTCTATATCTTGTGTTTTGTGGGTCGTGTTT3′
GPI2 Down RP	5′CACTGAAAAGAAAAGTGTATATTATCTTCTATAAGG3′
GPI2-URA3-PMET3 FP	5′AGTATAATTTTGAGTTTGTGTGTGTGTGTGTGGAAGTATTCTATATCTTGTGTTTTGTGGGTCGTGTCTAGAAGGACCACCTTTGATTG3′
GPI2-URA3-PMET3 RP	5′CATGCTGAAAATGGTTTGGGGGAGCTGAATCACTATCTTGCAAAATGGAAGAGCTTATATGTATTTCTTCCATTTTAATAAACGCGGATCC3′
GPI19-HIS1 FP	5′ATGATATTCCATTTTAACCAAAAAGAGAAAGCAGATTCAAGTAAATGTAAAACAATACATGTAAGAAAGACGGGGATCCTGGAGGATGAG3′
GPI19-HIS1 RP	5′TCATTCATATAGAACATCATTCACTAATGTAATAGGCAAATCCCAAACACCACTTGGTGCCTTATGAATACGGAATATTTATGAGAAACT3′
GPI19 Up FP	5′GAATTCGAAAACTAGATATTAATGTT3′
GPI19 Down RP	5′GTCGACTCTAGATCTCCTGCTTACTTTTTATTT3′
GPI15-HIS1 FP	5′ATGTCCTCATCGAAAAATTATAAATTGGAAATTTCCCCTAGTATCAACCAAACTAGTGCCACAGAATCGCGGGGATCCTGGAGGAATGAG3′
GPI15-HIS1 RP	5′CTAAATAACTTGTTTTAAACCTTGACCAGGCACTCTTCTCCAATAGCGTTTTGTGCTTCCAAATAGTAACGGAATATTTATGAGAAACT3′
GPI15 Up FP	5′TGGTAACCATTCATCAATCATATAG3′
GPI15 Down RP	5′GGTTTATACATAGATTTTATATACATTG3′
PACT1-GFP-RAS1 FP	5′ATTAGATATAATCAAAAAAATCAAAAAGAAACCCCGGGCAAACACAAATTCATATCCTCTAGAAGGACCACCTTTGATTG3′
PACT1-GFP-RAS1 RP	5′GGTTAAAGCGGATTTACCAACACCACCACCTCCAACAACAACTAATTTATATTCTCTCAATTTGTACAATTCATCCATAC3′
RAS1-HIS1 FP	5′ATGTTGAGAGAATATAAATTAGTTGTTGTTGGAGGTGGTGGTGTTGGTAAATCCGCTTTAACCATTCAACGGGGATCCTGGAGGATGAG3′
RAS1-HIS1 RP	5′TCAAACAATAACACAACATCCATTCTTTGATTTAGAGCTAGATTGTTTTTGACCAGAAAATTGATTTTCCGGAATATTTATGAGAAACT3′
RAS1-ARG4 FP	5′TTGATTCAATCCCATTTTGTTGATGAATATGACCCAACTATTGAGGATTCTTATCGTAAACAATGTACTTGTGGAATTGTGAGCGGAAG3′
RAS1-ARG4 RP	5′AGATTGTTGTTGTGGTTCTTGTTGTTGCTGTTGTTGTTGTCTTTGTGATTGTGATTGTGATTGATTTGGAATTTCCCAGTCACGACGTT3′
RAS1 FP	5′ATGTTGAGAGAATATAAATTAG3′
RAS1 RP	5′AACAATAACACAACATCCATTCT3′
RAS2-HIS1 FP	5′ATGTCATTACTACTAGATAGCTTACATGCATTAACAAAGAACTACGACATGTGTGTCATTGGCTCCAGTCGGGGATCCTGGAGGATGAG3′
RAS2-HIS1 RP	5′CTATGTTATTATGCAACATTTATTTTCAGCATGTATTTGACTGGATTTTGCCAGTGGGCCAGATGACTGCGGAATATTTATGAGAAACT3′
RAS2-ARG4 FP	5′AATGTCGGTAAATCAACCTTAGTCCTTCATTATGTGTATCATCATTTCGACGAAAGTTTGTATGACTTGTGTGGAATTGTGAGCGGAAG3′
RAS2-ARG4 RP	5′TTTTAATCGAGTTTTTCTTTGACGAGATGAAGTAGATGGTGTTTGTGCTATGGGTGTAGAACCATTCAAATTTTCCCAGTCACGACGTT3′
RAS2 Up FP	5′TCTAAATCCATAGTCTACATCAACTAATTT3′
RAS2 Down RP	5′AATAAGATTACATGTAACTCAAATTTTTGA3′
GPI3 FP	5′GCGGAGCTCATGGGATACAATATAGCAATGGTA3′
GPI3 RP	5′GAAGCTTCTAATTTACTTTGTTCGGAA3′
GPI3 5′hz RP	5′GGGCGAGATCTAGCAATTAGAAATTTCACTTTTGG3′
GPI33′hz FP	5′GGTCGACAGAAAATGTTTATAATTC3′
GPI3 Up FP	5′CACCACAAACGAATTACACAAGCA3′
GPI3 Down RP	5′ATAAATGGACTAAAAAGAAAGATCTG3′
RPS10 RP	5′TTTCTGGTGAATGGGTCAACGAC3′
PACT1 FP	5′GCGGGATCCGAGCTCCTCGATAATTCTAGTGGT3′
GAPDH RT FP	5′CAGCTATCAAGAAAGCTTCTG3′
GAPDH RT RP	5′GATGAGTAGCTTGAACCCAA3′
ERG11 RT FP	5′GGAACGAGATTGTAAGATAG3′
ERG11 RT RP	5′AGTACATTGGGTCATTTCAG3′
ERG1 RT FP	5′GGAACGAGATTGTAAGATAG3′
ERG1 RT RP	5′AGTACATTGGGTCATTTCAG3′
GPI2 RT FP	5′GGCCAATAGCATTTCTAAC3′
GPI2 RT RP	5′CCATCACAAAAACAGACAAA3′
GPI19 RT FP	5′CAAGAAGAAGAAGAAGGAGAA3′
GPI19 RT RP	5′AAACACCACTTGGTGCCTTA3′
GPI15 RT FP	5′CCACGATTATGGGCAGGTTA3′
GPI15 RT RP	5′CGCGGTAAGAATTCTGGAAA3′
GPI3 RT FP	5′TGCTGAACCGGAAGAAAACT3′
GPI3 RT RP	5′TACATCTTTGCAACCGCATC3′
ERG11 PR FP	5′ATGAAAGGGACCAATCTGTC3′
ERG11 PR RP	5′AAGTATTATCCTGTCTGATTCT3′
ERG1 PR FP	5′CATGTTTAAGAGACAAGTGTTT3′
ERG1 PR RP	5′TTCTCACCTCATCGTTCTCA3′