TABLE 5.
Percentage of different morphological forms observed after growing the strains in Spider medium at 37 °C for 120 min
GPI2 heterozygote (gpi2/GPI2) and conditional null GPI2 (gpi2/PMET3-GPI2) mutants in the different strain backgrounds as indicated showed lesser hyphae as compared with the wild type strains. The deletion of single alleles of GPI19 and GPI15 in the GPI2 heterozygous strain background does not affect hypha formation in the double heterozygote mutants GPI2/GPI19 (BWP17-gpi2/GPI2::gpi19/GPI19) and GPI2/GPI15 (BWP17-gpi2/GPI2::gpi15/GPI15). As published previously (6), the conditional null GPI19 mutant shows hyperfilamentation as compared with BWP17. However, disruption of a single GPI2 allele leads to reduced hyphae formation in the conditional null GPI19 mutant (BWP17-gpi19/PMET3-GFP-GPI19::gpi2/GPI2). Thus, reduced GPI2 levels seem to reduce filamentation in C. albicans. The experiment was done twice in duplicate.
| Yeast | Pseudo-hyphae | Hyphae | |
|---|---|---|---|
| % | % | % | |
| CAI4 | 12 ± 3 | 14 ± 4 | 74 ± 9 |
| CAI4-gpi2/GPI2 | 73 ± 6 | 18 ± 5 | 9 ± 4 |
| CAI4-gpi2/PMET3-GPI2 | 81 ± 9 | 17 ± 4 | 2 ± 2 |
| BWP17 | 10 ± 1 | 23 ± 4 | 67 ± 12 |
| BWP17-gpi2/GPI2 | 67 ± 9 | 19 ± 3 | 14 ± 2 |
| BWP17-gpi2/PMET3-GPI2 | 74 ± 8 | 16 ± 5 | 10 ± 3 |
| BWP17-gpi2/GPI2::gpi19/GPI19 | 69 ± 8 | 14 ± 2 | 17 ± 6 |
| BWP17-gpi2/GPI2::gpi15/GPI15 | 72 ± 7 | 16 ± 4 | 12 ± 4 |
| BWP17-gpi19/PMET3-GFP-GPI19 | 3 ± 1 | 3 ± 2 | 94 ± 8 |
| BWP17-gpi19/PMET3-GFP-GPI19::gpi2/GPI2 | 58 ± 12 | 24 ± 7 | 18 ± 4 |