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. 2013 Dec 16;289(6):3432–3443. doi: 10.1074/jbc.M113.529131

FIGURE 5.

FIGURE 5.

Differential cytokine responses to C. albicans (Ca) hyphal versus yeast glucan is mediated through a Dectin-1-dependent mechanism. A, hyphal glucan stimulates cytokine expression from normal human PBMCs, whereas yeast glucan does not. B, PBMCs from Dectin-1-deficient patients do not respond to C. albicans hyphal or yeast glucan, indicating that the hyphal glucan response is Dectin-1-dependent. C, C. albicans hyphal glucan stimulates cytokine expression in human monocyte-derived-macrophages. Monocyte-derived macrophages were incubated with hyphal or yeast glucan. Media alone served as control. Supernatant was collected for TNFα, IL-6, and IL-1β ELISA after 24 h stimulation. *, p < 0.05 versus RPMI control. D, C. albicans hyphal glucan is a more potent stimulus for IL-1β production in human monocyte-derived macrophages than yeast glucan. IL-1β mRNA expression was measured by RT-PCR after 4 h of stimulation. Extracellular and intracellular IL-1β production was measured by ELISA after 24 h of stimulation. Data are presented as the mean ± S.E. Results are from at least three sets of experiments, with a minimum of six volunteers were pooled and analyzed using GraphPad Prism software. *, p < 0.05.