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. 2013 Dec 19;289(6):3444–3456. doi: 10.1074/jbc.M113.507541

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FIGURE 1. — C. elegans homologs of WDR-20, WDR-48, and USP-46 interact in vitro. A, domain conservation between the human and C. elegans WD-40-repeat proteins WDR-20 and WDR-48. WD-40 domains were assigned using ProSite and SMART (String Matching Algorithms Research Tool). B, representative immunoblot (IB) analysis of the immunoprecipitation of HA·WDR-20 and Myc·WDR-48 by FLAG·USP-46 from HEK293T cells. FLAG·USP-46 was expressed alone (lanes 1 and 2) or in the presence of HA·WDR-20 and/or Myc·WDR-48 (lanes 3–5), and the resulting isolated complexes were assayed by immunoblot analysis. GFP is included as an expression and loading control for each condition. Controls are shown for antibody specificity (lane 1) and background binding (lane 6). Similar results were obtained in more than five independent experiments.