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Cytotoxic activity of EcA and variants on MV4:11 (A), Ramos (B), and PBMCs (C) are shown. MV4:11, Ramos, and PBMCs were treated with different concentrations of enzymes for 96 h. Cells viability was determined by the MTT assay. D, trypan blue assay. MV4:11 cells were treated with 0.8 units/ml of enzymes for 96 h. Cell viability was determined by trypan blue exclusion assay. Each experiment was performed three times; mean ± S.D. are shown. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
