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Cell cycle studies of MV4:11 cells after treatment with EcA variants
The cells were treated with 0.8 units/ml EcA variants for 96 h. Flow cytometric analysis was performed by analyzing 10,000 gated cells using FACS Diva software (BD Biosciences).
| EcA variants | Percentage of cell arrested |
|||
|---|---|---|---|---|
| G0 | G1 | S | G2/M | |
| Control | 3.8 | 73.2 | 9.7 | 13.4 |
| WT | 45.9 | 49.1 | 2.8 | 2.1 |
| K288S | 60.5 | 37.4 | 1.5 | 0.6 |
| K288R | 63.3 | 34.2 | 2.1 | 0.4 |
| K288R/Y176F | 58.2 | 38.9 | 2.2 | 0.6 |
