TABLE 1.
Biacore measurement of anti-TuAg (clone H158) IgG1 variants binding to monomeric human FcγRIIA (Arg-131), FcγRIIA (His-131), FcγRIIB and FcγRIIIA (Val-158), and FcγRIIIA (Phe-158)
| Antibody | Type | Sequence variations |
FcγR (KD) (μm) |
|||||
|---|---|---|---|---|---|---|---|---|
| Fc chain A | Fc chain B | IIA 131R | IIA 131H | IIB | IIIA 158V | IIIA 158F | ||
| Anti-TuAg IgG1 (WT) | Homodimer | No change | No change | 9.2 | 6.11 | 13.9 | 1.64 | 7.56 |
| Anti-TuAg IgG1 (3×) | Homodimer | S239D + A330L + I332E | S239D + A330L + I332E | 9.8 | 6.98 | 6.9 | 0.046 | 0.059 |
| Anti-TuAg IgG1 (V1) | Heterodimer | S239D + S298A + E356K + D399K | A330L + I332E + K392D + K409D | 6.71 | 5.53 | 12.4 | 0.028 | 0.04 |
| Anti-TuAg IgG1 (V2) | Heterodimer | S239D + S298A + K392D + K409D | A330L + I332E + E356K + D399K | 7.5 | 5.82 | 14.7 | 0.03 | 0.047 |
| Anti-TuAg IgG1 (V3) | Heterodimer | S239D + S298A + E356K + D399K | S239D + A330L + I332E + K392D + K409D | 6.9 | 5.23 | 8.6 | 0.019 | 0.022 |
| Anti-TuAg IgG1 (V4) | Heterodimer | S239D + S298A + K392D + K409D | S239D + A330L + I332E + E356K + D399K | 3.88 | 2.96 | 6.3 | 0.025 | 0.031 |