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. 2013 Dec 20;289(6):3703–3712. doi: 10.1074/jbc.M113.538264

FIGURE 3.

FIGURE 3.

U1A binds directly to the SMN 3′-UTR with high affinity and specificity. Gel shift assays of radiolabeled SMN RNA incubated with recombinant U1A protein were performed. A, 10-fold titrations of U1A protein with SMN wild type 3′-UTR. B, 10-fold dilutions of U1A protein with SMN site 1 mutant. C, 10-fold dilutions of U1A with the SMN site 1 and site 2 mutants. D, 2-fold dilutions of U1A protein with SMN wild type 3′-UTR under equilibrium conditions. E, 2-fold dilutions of U1A protein with SMN site 1 mutant under equilibrium conditions. F, binding curve of experiment in D with Kd.