Fig. 2A–B.

Characterization of human synovial sarcoma cell lines GUSS-1, GUSS-2, and GUSS-3. We performed reverse transcription–PCR on total RNA from each of our synovial sarcoma cell lines using the SS18, SSX (consensus), SSX1, and SSX2 primers. The PCR products were then separated by electrophoresis on an agarose gel and visualized with ethidium bromide. All cell lines are of the SS18-SSX1 subtype (A). Protein lysates from each were analyzed by Western blot for SS18-SSX1 expression. All cell lines express SS18-SSX1 protein (B). G1 = GUSS-1; G2 = GUSS-2; G3 = GUSS-3 prechemotherapy; G3b = GUSS-3 postchemotherapy; IgG = negative IgG control. Actin was used as a protein concentration loading control.