Fig. 4A–C.

Successful reduction of SS18-SSX1 protein by shRNA. Protein lysates were extracted from synovial sarcoma cell lines transfected with anti-SS18-SSX shRNAs. (A) GUSS-1; (B) GUSS-2; (C) GUSS-3. U = untreated cells; VC = empty vector control; SC = shRNA scramble control; T = treated cells. Numbers to the right of the panel quantify percent reduction in protein expression by densitometry analysis. Variable knockdown of SYT-SSX was seen with various shRNA constructs, but > 90% reduction was seen in all three cell lines with shRNAs 1, 5, and 6.