Fig. 6A–B.

SS18-SSX knockdown induces apoptosis. Caspase-3 activity was measured after disruption of SS18-SSX (A). We saw a significant fold increase in caspase-3 activity in all three cell lines with each of the shRNA constructs. Untreated cells (U), empty vector (VC), and scramble shRNA (SC) were used as negative controls. These results depict the average of three separate experiments, each run in triplicate (n = 9). Total cell lysates were analyzed by Western blot analysis for full-length PARP and cleaved PARP. Cells treated with anti-SS18-SSX shRNA expressed cleaved PARP, whereas untreated cells did not. G1 = GUSS-1; G2 = GUSS-2; G3 = GUSS-3.