Fig. 7A–B.

Reinsertion of exon-8 into synovial sarcoma cell lines reduces cell viability without affecting SS18-SSX expression. Synovial sarcoma cell lines were transfected with a pcDNA4TO vector containing the exon-8 sequence. Cell viability was examined by WST-1 analysis. We saw a significant reduction in cell viability after the exon-8 peptide was reintroduced into the cell (A). SS18-SSX expression was examined by Western blot analysis (B). There was no change in SS18-SSX expression after reinsertion of exon-8 into the cell lines. Actin was used as a protein loading control. S1 = GUSS-1; S2 = GUSS-2; S3 = GUSS-3. Positive FLAG expression confirms the presence of exon-8. U = untreated cells; VC = empty vector control. A depicts the results of three separate experiments each run in triplicate (n = 9).