Fig. 4.

Schematic diagram, confirmation, and validation of ApoE capture antibody-conjugated functional beads using FACS analysis. A, the schematic process of target protein detection by FACS analysis using capture antibody-conjugated functional beads. B, the attachment of ApoE capture antibody to functional beads was demonstrated by PE-conjugated anti-mouse IgG treatment. Negative controls were compared, including naked beads, no antibody incubation, and PE-conjugated anti-rabbit IgG incubation. C, D, validation of ApoE capture antibody-conjugated functional beads was performed using serial dilution of calibrator serum (C) and ApoE standard protein (D), showing proportional change of MFI in an ApoE concentration-dependent manner.