Fig. 5.

Proteome profiling and Western blot analyses. A, Mitochondrial proteins of the wild-type (WT) and sector (Sec) prepared from two independent experiments were analyzed by SDS-PAGE and silver staining. B, Heat map analysis of the WT and Sec mitochondrial protein expression profiles. Int, intensity of protein peak. C, Western blot analysis of the WT and Sec mitochondrial and whole cell protein samples using different antibodies. Asp B, septum-specific protein septin B; Per, lipid droplet-specific perilipin-like protein; Hsp70, heat shock protein Hsp70; LetM1, calcium antiporter LetM1; Gpx, glutathione perioxidases; CycA, cytochrome c protein.