Fig. 8.

PI3K/Akt inhibition modulates the invasive ability and chemosensitivity of MHCC97H cells both in vitro and in vivo. A, the MHCC97H cells were pretreated with wortmannin or Akt shRNA. The expression of PI3K/Akt/NF-κB signaling molecules was then examined via Western blot analysis. B, wortmannin or Akt shRNA treatment decreased the invasive ability of MHCC97H cells in vitro. C and D, wortmannin or Akt shRNA treatment also alleviated the chemoresistance of MHCC97H cells, as revealed in vitro and in vivo. E, down-regulation of PI3K/Akt/NF-κB signaling molecules was also shown by IHC staining in xenograft tumors derived from wortmannin or Akt shRNA treatment cells (400×). *p < 0.05 versus DMSO treatment cells; ^#p < 0.05 versus control siRNA treatment cells. The data are means ± S.D. of three independent assays.