Skip to main content
. 2013 Nov 19;13(2):520–536. doi: 10.1074/mcp.M113.034025

Table I. qRT-PCR conditions and primer sequences for analysis of gene expression.

Gene Primers Amplicon (bp)
ST3GAL1 5′-CAGAGATGGACGGTCACT-3′; 5′-CAACTGTGGTTTCTGACG-3′ 197
ST3GAL2 5′-GTGCCTCCGACTGGTTTG-3′; 5′-GAAGCGGTAGGGGTTCTC-3′ 191
ST3GAL3 5′-TATGCTTCAGCCTTGATG-3′; 5′-TTGGTGACTGACAAGATGG-3′ 164
ST3GAL4 5′-ATGTTGGCTCTGGTCCTG-3′; 5′-AGGAAGATGGGCTGATCC-3′ 176
ST3GAL5 5′-CAAAGCAAGATGAGAAGG-3′; 5′-AAACTTGGGACGACATTC-3′ 213
ST3GAL6 5′-ATGTCTATTGGGTGGCAC-3′; 5′-CGCACACAGAAAAGGGTG-3′ 189
ST6GAL1 5′-CTTGTTTTCCTGCTCAGA-3′; 5′-GCAAACAGAAGAAAGACCA-3′ 166
ST6GAL2 5′-ACGCTGCTGATTGACTCTTCT-3′; 5′-CACATACTGGCACTCATCTAA-3′ 160
ST6GALNAC1 5′-CTGGTCTTCTTTCTCTTCG-3′; 5′-GTTGAGGGCATTGTTCTCT-3′ 192
ST6GALNAC2 5′-CTTTGCCCTGTACTTCTCG-3′; 5′-CAGCACTGGAATGGAGAGA-3′ 205
ST6GALNAC3 5′-GGACAACCTGGTACAAAGT-3′; 5′-TATCTCATTTCCCACCTTC-3′ 174
ST6GALNAC4 5′-ACCTGCCTGGACCACCACT-3′; 5′-TCGGCACTGTCGATCTCAG-3′ 188
ST6GALNAC5 5′-TGGACGGATACCTCGGAGT-3′; 5′-GTCTGGTCAATCTGGGAGC-3′ 121
ST6GALNAC6 5′-ACCTACCCCTCAGCAGACG-3′; 5′-CTTGAGGTTGACAGGTCGG-3′ 179
ST8SIA1 5′-TACTCTCTCTTCCCACAGG-3′; 5′-GACAAAGGAGGGAGATTGC-3′ 149
ST8SIA2 5′-GTGGTCTTCCTCATCTTCG-3′; 5′-GAGGAGCCGTTTATTACAAC-3′ 140
ST8SIA3 5′-ATTCTCTCACCCAGGAACTC-3′; 5′-CAATCCGAACACTATTCTTG-3′ 141
ST8SIA4 5′-CAAGAACTGAGGAGCACC-3′; 5′-TTTCCAACCTTCTACATTGTG-3′ 140
ST8SIA5 5′-CCTTTGCCTTGGTGACCT-3′; 5′-CATGGACAGCACCTTCACT-3′ 152
ST8SIA6 5′-CGGCAAGCAGAAGAATATG-3′; 5′-GCTTTCCACCTCGTAACTC-3′ 126
GAPDH 5′-CTCCTCCACCTTTGACGCTG-3′; 5′-TCCTCTTGTGCTCTTGCTGG-3′ 175