Table III. N-glycans analyzed in MHCC97H and MHCC97L cell lines via MALDI-TOF-MS.
Peak number | Observed m/z |
Composition |
||||||
---|---|---|---|---|---|---|---|---|
MHCC97H | MHCC97L | Hex | HexNAc | Man | GlcNAc | NeuAc | Deoxyhexose | |
1 | 1345.80 | 1345.65 | 0 | 0 | 3 | 2 | 0 | 1 |
2 | 1375.88 | 1375.63 | 1 | 0 | 3 | 2 | 0 | 0 |
3 | 1416.85 | 1416.77 | 0 | 1 | 3 | 2 | 0 | 0 |
4 | 1579.88 | 1579.73 | 0 | 0 | 5 | 2 | 0 | 0 |
5 | 1783.98 | 1783.83 | 0 | 0 | 6 | 2 | 0 | 0 |
6 | 1836.07 | 1835.77 | 0 | 2 | 3 | 2 | 0 | 1 |
7 | 1988.07 | 1987.92 | 0 | 0 | 7 | 2 | 0 | 0 |
8 | 2156.15 | 2155.95 | 1 | 1 | 3 | 2 | 1 | 1 |
9 | 2186.17 | No | 2 | 1 | 3 | 2 | 1 | 0 |
10 | 2192.14 | 2191.95 | 0 | 0 | 8 | 2 | 0 | 0 |
11 | 2244.28 | 2243.99 | 2 | 2 | 3 | 2 | 0 | 1 |
12 | 2360.17 | 2360.07 | 2 | 1 | 3 | 2 | 1 | 1 |
13 | 2396.20 | 2396.00 | 0 | 0 | 9 | 2 | 0 | 0 |
14 | 2431.20 | 2431.00 | 2 | 2 | 3 | 2 | 1 | 0 |
15 | 2564.27 | 2564.05 | 3 | 1 | 3 | 2 | 1 | 1 |
16 | 2605.25 | 2605.04 | 2 | 2 | 3 | 2 | 1 | 1 |
17 | 2693.25 | 2693.03 | 3 | 3 | 3 | 2 | 0 | 1 |
18 | 2779.32 | 2779.10 | 2 | 2 | 3 | 2 | 1 | 2 |
19 | 2792.33 | 2792.09 | 2 | 2 | 3 | 2 | 2 | 0 |
20 | 2880.23 | 2880.07 | 3 | 3 | 3 | 2 | 1 | 0 |
21 | 2938.25 | 2938.03 | 3 | 4 | 3 | 2 | 0 | 1 |
22 | 2966.34 | 2966.08 | 2 | 2 | 3 | 2 | 2 | 1 |
23 | 3054.38 | 3054.15 | 3 | 3 | 3 | 2 | 1 | 1 |
24 | 3142.43 | 3142.26 | 4 | 4 | 3 | 2 | 0 | 1 |
25 | 3415.52 | 3415.41 | 3 | 3 | 3 | 2 | 2 | 1 |
26 | 3503.43 | 3503.03 | 4 | 4 | 3 | 2 | 1 | 1 |
27 | 3602.31 | No | 3 | 3 | 3 | 2 | 3 | 0 |
28 | 3777.36 | 3776.20 | 3 | 3 | 3 | 2 | 3 | 1 |
29 | 3864.49 | 3864.92 | 4 | 4 | 3 | 2 | 2 | 1 |
30 | 3952.45 | 3952.04 | 5 | 5 | 3 | 2 | 1 | 1 |
31 | 4226.32 | 4225.85 | 4 | 4 | 3 | 2 | 3 | 1 |
32 | 4314.22 | 4314.00 | 5 | 5 | 3 | 2 | 2 | 1 |
33 | 4586.98 | 4586.58 | 4 | 4 | 3 | 2 | 4 | 1 |
Notes: The N-glycans were observed as [M+Na]+.
Hex, hexose; HexNAc, N-acetylhexosamine; Man, mannose; GlcNAc, N-acetylglucosamine; NeuAc, N-acetylneuraminic acid.