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. 2013 Dec 3;13(2):551–565. doi: 10.1074/mcp.M113.034058

Fig. 4.

Fig. 4.

Multiple SCD1 TQ motifs are required for efficient Rad53 priming and autoactivation when either Mec1 adaptor is abolished. A, C, relative S350 and T354 phosphorylation levels in the indicated strains. B, effect of rad9Δ or mrc1Δ on the relative levels of monophosphorylated rad53-T8-3AQ1–17 fragment MENIAQPTQQSAQAAQR in Rad53T8-3AQ. See supplemental Figs. S4B and S4C for the raw extracted ion chromatogram data used for quantitation. D, effect of rad9Δ or mrc1Δ on the relative levels of mono- (a) and diphosphorylated (b) rad53-T5T8-2AQ1–17 fragment MENITQPTQQSAQAAQR in Rad53T5T8-2AQ. See supplemental Fig. S4D for the raw extracted ion chromatogram data used for quantitation.