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Lectin affinity enrichment and identification of N-glycoproteins from tomato fruit pericarp. A, SYPRO ruby stained SDS-PAGE gel of proteins bound to each lectin (ConA, GNA, and LCH). B, identification of glycoproteins using a shotgun MS/MS approach. The numbers of predicted N-glycosylation sites in each of the lectin binding glycoprotein fractions are indicated. C, Venn diagram of the glycoproteins identified through shotgun sequencing associated with each of the three lectins.
