Fig. 5.

Transient expression and purification of recombinant xyloglucan-specific fungal endoglucanase inhibitor protein (XEGIP). A, Western blot analysis (upper panel) using an anti-myc antiserum of proteins extracted from N. benthamiana leaves transiently expressing recombinant XEGIP fused to a Myc tag. Protein samples were extracted 2, 3, and 4 days post infiltration (DPI). Lower panel shows an image of the RuBisCO large subunit (RbcL) protein in an SDS-PAGE gel stained with Coomassie brilliant blue (CBB), used as a loading control. B, SDS-PAGE gel (stained with CBB) of total protein (TP) from N. benthamiana leaves and a sample of the recombinant XEGIP+TAP after acidic elution from IgG beads, appearing as a double band (arrow).