Table II. N-glycans identified in the recombinant xyloglucan-specific endoglucanase inhibitor protein (XEGIP).
| Peptide sequence | Glycan composition | Mass of glycan | RT (min) | m/z | z | M+H | Peptide+H |
|---|---|---|---|---|---|---|---|
| ELANVTR | (Hex)4(HexNAc)2 | 1054.36 | 10.97 | 928.90 | 2 | 1856.80 | 802.44 |
| ELANVTR | (Hex)5(HexNAc)2 | 1216.36 | 10.97 | 1009.90 | 2 | 2018.80 | 802.44 |
| ELANVTR | (Hex)6(HexNAc)2 | 1378.36 | 10.97 | 1090.90 | 2 | 2180.80 | 802.44 |
| ELANVTR | (Hex)7(HexNAc)2* | 1540.36 | 10.97 | 1171.90 | 2 | 2342.80 | 802.44 |
| ELANVTR | (Hex)8(HexNAc)2* | 1702.36 | 10.97 | 835.60 | 3 | 2504.80 | 802.44 |
| ELANVTR | (Hex)9(HexNAc)2* | 1863.76 | 10.97 | 889.4 | 3 | 2666.20 | 802.44 |
| QNQTSFRPK | (Hex)6(HexNAc)2 | 1378.12 | 11.36 | 822.80 | 3 | 2466.40 | 1088.28 |
| QNQTSFRPK | (Hex)7(HexNAc)2 | 1540.12 | 11.36 | 876.80 | 3 | 2628.40 | 1088.28 |
| QNQTSFRPK | (Hex)8(HexNAc)2* | 1702.12 | 11.36 | 930.80 | 3 | 2790.40 | 1088.28 |
| QNQTSFRPK | (Hex)9(HexNAc)2* | 1540.12 | 11.36 | 984.80 | 3 | 2952.40 | 1088.28 |
| VVPINTTLLSIDNQGVGGTK | (Hex)4(HexNAc)2 | 1054.08 | 21.49 | 1027.40 | 3 | 3080.20 | 2026.12 |
| VVPINTTLLSIDNQGVGGTK | (Hex)5(HexNAc)2 | 1216.08 | 21.49 | 1081.40 | 3 | 3242.20 | 2026.12 |
| VVPINTTLLSIDNQGVGGTK | (Hex)6(HexNAc)2 | 1378.38 | 21.49 | 1135.50 | 3 | 3404.50 | 2026.12 |
| VVPINTTLLSIDNQGVGGTK | (Hex)7(HexNAc)2* | 1540.68 | 21.49 | 1189.60 | 3 | 3566.80 | 2026.12 |
| VVPINTTLLSIDNQGVGGTK | (Hex)8(HexNAc)2* | 1702.68 | 21.49 | 1243.60 | 3 | 3728.80 | 2026.12 |
| VVPINTTLLSIDNQGVGGTK | (Hex)9(HexNAc)2 | 1864.08 | 21.49 | 1297.40 | 3 | 3890.20 | 2026.12 |
| QTTCANFNFTSIDdpaflyk | (Hex)5(HexNAc)2 | 1216.42 | 25.57 | 1190.50 | 3 | 3569.50 | 2353.08 |
| QTTCANFNFTSIDdpaflyk | (Hex)6(HexNAc)2 | 1378.12 | 25.57 | 1244.40 | 3 | 3731.20 | 2353.08 |
| QTTCANFNFTSIDdpaflyk | (Hex)7(HexNAc)2 | 1540.72 | 25.57 | 1298.60 | 3 | 3893.80 | 2353.08 |
| QTTCANFNFTSIDdpaflyk | (Hex)8(HexNAc)2a | 1702.12 | 25.57 | 1352.40 | 3 | 4055.20 | 2353.08 |
| QTTCANFNFTSIDdpaflyk | (Hex)9(HexNAc)2a | 1864.42 | 25.57 | 1406.50 | 3 | 4217.50 | 2353.08 |
a High abundant glycoforms. The N-glycans and glycoforms were identified by nanoLC-PI scan (+204) triggered IDA analysis. Lower case letters in the peptide sequences refer to amino acid residues in the TAP tag fusion.