Fig. 2.

Use of ditelocentric TDF-J lines to map VRN-D4. a C-Banding of chromosome 5D, telocentric chromosomes 5DS and 5DL and isochromosome iso5D. b Heading time of lines carrying different dosages of the 5DS arms: 0 copies (mt5DL, dt5DL and nullisomics 5D), one copy (mt5DS), two copies (dt5DS and TDF-J), and five copies (dt-iso5DS + 5D). The number of plants in each class is indicated at the base of the bar and the standard error of the mean in the error bars. The P values are the results of two t tests for samples with unequal variances on data transformed to restored normality of residuals (Shapiro–Wilk test not significant). The dt-iso-5DS + 5D line was not compared statistically because a single plant was obtained in this class. Note the acceleration of flowering with increase dosage of the 5DS chromosome arm