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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Cancer. 2013 Oct 25;120(4):480–491. doi: 10.1002/cncr.28453

Fig. 2. RF treatment induced autophagy but not apoptosis in pancreatic cancer cells.

Fig. 2

Panc-1, MiaPaCa-2 and HPDE cells were RF treated as described in Fig. 1 for 1-5 min and stained with (A) anti-LC3B antibody (red) to assess autophagy and (B) anti-cleaved caspase 3 antibody (red) to assess apoptosis. GCB treatment at 2 μM was used as a positive control for cleaved caspase 3. Cells were stained with FITC-conjugated phalloidin (green) as a cytoskeleton marker, and with DAPI (blue) as a nuclear marker. Phalloidin staining was not done in Fig. 2B. Magnification 60x. Scale bar is 10 μm.