Fig. 4. Combination treatment with GCB and RF field exposure produced greater pancreatic cancer cell death than does either treatment alone.

(A) MiaPaCa-2 or (B) Panc-1 cells were treated with GCB at the dosages noted for 24 h prior to RF exposure, which lasted for 4 min, and with the same dose of GCB alone or RF alone. Untreated cells were used as controls. Cells were fixed and analyzed 24 h after treatment by FACS using an Annexin V/PI kit as described in Fig.1