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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Cancer. 2013 Oct 25;120(4):480–491. doi: 10.1002/cncr.28453

Fig. 5. Autophagy induction in pancreatic cancer cells after RF treatment was extended when combined with GCB.

Fig. 5

(A) Panc-1 or (B) MiaPaCa-2 cells were treated with 0.2 or 0.005 μM of GCB, respectively, for 24 h and then exposed to the RF field (900W, 13.56 MHz) for 4 min. Cells were fixed 24, 46, 72, or 96 h after RF treatment and stained with antibodies against LC3B or cleaved caspase 3 (both red), phalloidin (green), and DAPI (blue). Untreated cells and cells treated with RF or GCB alone were used as controls.