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The primer and TaqMan probe sequences used in this study
| Genes |
Forward primer sequence |
Probe sequence |
Reverse primer sequence |
Amplification |
|---|---|---|---|---|
| (5′ → 3′) | (5′ → 3′) | (5′ → 3′) | Efficiency (%) | |
|
MT-ND1 |
CCCCTAAAACCCGCCACATC |
6FAM-ACCCTCTACATCACCGCCCCGACC-TAMRA |
GTAGAAGAGCGATGGTGAGAGC |
94.6 |
| β-actin | TCACCCACACTGTGCCCATCTACGA | 6FAM-ATGCCCTCCCCCATGCCATCC-TAMRA | TCGGTGAGGATCTTCATGAGGTA | 95.2 |
