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. 2013 Jul 12;200(3):796–807. doi: 10.1111/nph.12405

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© 2013 The Authors New Phytologist © 2013 New Phytologist Trust

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Correlative transmission electron micrograph (TEM) and nano-scale secondary ion mass spectrometry (NanoSIMS) imaging after 5 min of exposure of wheat (Triticum aestivum) to ¹⁵N-labelled . (a) TEM of the rhizosphere (rh), showing bacteria (b) adjacent to a root cell (c), root hair (r) and soil particles (s). (b) ¹²C¹⁴N⁻ NanoSIMS image of the same region, highlighting the bacteria and organic materials including extracellular mucilage (e) associated with soil particles and bacteria. (c) ¹⁶O⁻ NanoSIMS image of the same region highlighting soil particles containing oxides. (d) Hue-Saturation-Intensity (HSI) image generated from the NanoSIMS ¹⁵N : ¹⁴N ratio of the same region, showing ¹⁵N : ¹⁴N ratio intensities from natural abundance (blue, 0.0037) to enriched (pink, 0.30). Bar, 1 μm for all images.

Inline graphic — Correlative transmission electron micrograph (TEM) and nano-scale secondary ion mass spectrometry (NanoSIMS) imaging after 5 min of exposure of wheat (Triticum aestivum) to ¹⁵N-labelled . (a) TEM of the rhizosphere (rh), showing bacteria (b) adjacent to a root cell (c), root hair (r) and soil particles (s). (b) ¹²C¹⁴N⁻ NanoSIMS image of the same region, highlighting the bacteria and organic materials including extracellular mucilage (e) associated with soil particles and bacteria. (c) ¹⁶O⁻ NanoSIMS image of the same region highlighting soil particles containing oxides. (d) Hue-Saturation-Intensity (HSI) image generated from the NanoSIMS ¹⁵N : ¹⁴N ratio of the same region, showing ¹⁵N : ¹⁴N ratio intensities from natural abundance (blue, 0.0037) to enriched (pink, 0.30). Bar, 1 μm for all images.