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. 2013 Jul 12;200(3):796–807. doi: 10.1111/nph.12405

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© 2013 The Authors New Phytologist © 2013 New Phytologist Trust

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Nano-scale secondary ion mass spectrometry (NanoSIMS) imaging and analysis of wheat (Triticum aestivum) roots in situ within the embedded soil core after 5 min of exposure to ¹⁵N-labelled glutamate or . Examples of the location of cellular regions analysed within a root are depicted in Fig. 4(c). Root and cell structure is visualized in ¹²C images (a, b; g, h). Enrichment levels of ¹⁵N within these regions are shown as ¹⁵N : ¹⁴N Hue-Saturation-Intensity (HSI) images (c, d; i, j). The HSI colour scale (j; 0.0037–0.03; ¹⁵N : ¹⁴N natural abundance = 0.0037) applies to all HSI images. Line scans show numerical levels of ¹⁵N enrichment across the subcellular regions (e, f; k, l), with data acquired from the lines indicated on each of the respective ¹⁵N : ¹⁴N HSI images. High levels of ¹⁵N enrichment can be seen in the treatment within distinct cellular regions, whereas the ¹⁵N-glutamate treatment shows cells with very low levels of ¹⁵N enrichment just above natural abundance. Bar, 5 μm for all images.

Inline graphic — Nano-scale secondary ion mass spectrometry (NanoSIMS) imaging and analysis of wheat (Triticum aestivum) roots in situ within the embedded soil core after 5 min of exposure to ¹⁵N-labelled glutamate or . Examples of the location of cellular regions analysed within a root are depicted in Fig. 4(c). Root and cell structure is visualized in ¹²C images (a, b; g, h). Enrichment levels of ¹⁵N within these regions are shown as ¹⁵N : ¹⁴N Hue-Saturation-Intensity (HSI) images (c, d; i, j). The HSI colour scale (j; 0.0037–0.03; ¹⁵N : ¹⁴N natural abundance = 0.0037) applies to all HSI images. Line scans show numerical levels of ¹⁵N enrichment across the subcellular regions (e, f; k, l), with data acquired from the lines indicated on each of the respective ¹⁵N : ¹⁴N HSI images. High levels of ¹⁵N enrichment can be seen in the treatment within distinct cellular regions, whereas the ¹⁵N-glutamate treatment shows cells with very low levels of ¹⁵N enrichment just above natural abundance. Bar, 5 μm for all images.