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. 2013 Jul 12;200(3):796–807. doi: 10.1111/nph.12405

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© 2013 The Authors New Phytologist © 2013 New Phytologist Trust

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Nano-scale secondary ion mass spectrometry (NanoSIMS) derived ¹⁵N : ¹⁴N isotopic ratios for different wheat (Triticum aestivum) root tissues (intercellular cortex, endodermis and vascular bundle) after injection of (closed circle) or ¹⁵N-glutamate (open circle) into the rhizosphere. Dotted lines represent ¹⁵N natural abundance. Values represent mean ± SE (n = 13 and n = 10 for roots in the and glutamate treatments, respectively). The dotted line represents ¹⁵N natural abundance. Significant differences between the ¹⁵NH₄ and ¹⁵N-glutamate treatments: ***, P < 0.001.

Inline graphic — Nano-scale secondary ion mass spectrometry (NanoSIMS) derived ¹⁵N : ¹⁴N isotopic ratios for different wheat (Triticum aestivum) root tissues (intercellular cortex, endodermis and vascular bundle) after injection of (closed circle) or ¹⁵N-glutamate (open circle) into the rhizosphere. Dotted lines represent ¹⁵N natural abundance. Values represent mean ± SE (n = 13 and n = 10 for roots in the and glutamate treatments, respectively). The dotted line represents ¹⁵N natural abundance. Significant differences between the ¹⁵NH₄ and ¹⁵N-glutamate treatments: ***, P < 0.001.