Figure 1.
SDS–PAGE and immunoblotting of protein extracts from somatic tissues, reproductive tissues, and spermatogenic cells. Immunoblotting with protein extracts from brain, heart, kidney, liver, lung, skeletal muscle, spleen, ovary, and cauda epididymal sperm (A). Immunoblotting with protein extracts from testis, pachytene spermatocytes, round spermatids, condensing spermatids, caput epididymal sperm, and cauda epididymal sperm (B). The somatic-type TPI1 protein (Mr 27,700) was detected in all protein samples examined, except cauda epididymal sperm, using an anti-TPI1 antibody. In spermatogenic cells and sperm, two male germ line-specific TPI1 bands (Mr 33,400 and 30,800) were present.