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In vitro dephosphorylation of TPI1 in mouse sperm. CIP treatment of testis and cauda epididymal sperm proteins probed with anti-TPI1 antibody (A) and cauda epididymal sperm proteins probed with anti-AKAP4 antibody as a positive control (B). The two arrows point to the two AKAP4 forms present prior to dephosphorylation. In vitro dephosphorylation analysis indicated that the two male germ line-specific TPI1 isozymes did not result from differences in phosphorylation.
