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SDS–PAGE and immunoblotting of detergent-extracted sperm proteins. Immunoblotting on SDS-insoluble head and tail proteins, Triton X-100-soluble and -insoluble, whole sperm proteins, and Triton X-100/S-EDTA-soluble and -insoluble whole sperm proteins was performed. The two male germ line-specific bands (Mr 33,400 and 30,800) were enriched in the S-EDTA-treated sperm tail fraction, in Triton X-100-insoluble sperm proteins, and in Triton X-100/S-EDTA-insoluble sperm proteins.
